cytotoxic effect of a new endodontic cement and mineral trioxide aggregate on l929 line culture

introduction : the aim of this study was to compare the cytotoxicity of mineral trioxide aggregate (mta) and a new endodontic cement (nec) on l929 mouse fibroblasts. materials and methods: different dilutions (neat, 1/2, 1/10, 1/100) of fresh and set materials placed adjacent flasks of l929 in dmem medium. cellular viability was assessed using mtt assay in three time intervals (24, 48, and 72 h after mixing). differences in mean cell viability values between materials were assessed by using the one-way anova and bonferoni post-test. optical microscopic analysis of morphology of the untreated control and the cement-treated cell cultures were carried out in all experimental periods. results: it was indicated that there was not a significant difference in cytotoxicity among the materials of test and between them and the control group. however, there was a statistically significant difference between different time intervals within each group (p< 0.05) and between different concentration of test materials (p<0.05). in all samples, set materials showed better viability than fresh ones. conclusion: according to results of this study, nec and mta have similar cytotoxic effect on l929 cell culture.